- B.A., Chemistry, Alfred University, 1968
- M.D., Medicine, Albert Einstein College of Medicine, 1975
- Ph.D., Cell Biology, Albert Einstein College of Medicine, 1975
Coronavirus replication and pathogenesis.
The replication of the murine coronavirus MHV (mouse hepatitis virus) and the molecular and genetic basis of its interactions with the host are being studied. MHV produces a broad spectrum of disease in the mouse, and provides excellent small animal models for hepatitis, for SARS, and for multiple sclerosis.
We have three projects in the laboratory. Using an MHV model for SARS, we are using a genetic approach to determine the role of various coronavirus proteins in the pathogenesis of SARS. The second project is focused on understanding how cis-acting sequences at the 5' and 3' untranslated regions (UTRs) function in replication.using biochemical, cell biologic, and reverse genetic approaches. The third project is investigating the role that molecular mimicry of the murine Fc receptor by the MHV spike protein has on the pathogenesis in a CNS disease model.
Liu, P., Li, L., Keane, S.C., Yang, D., Leibowitz, J.L., and Giedroc, D.P. 2009. Mouse hepatitis virus stem-loop 2 adopts a uYNMG(U)a-like tetraloop structure that is highly functionally tolerant of base substitutions. J Virol 83: 12084-12093.
Li, L., Kang, H., Liu, P., Makkinje, N., Williamson, S.T., Leibowitz, J.L., and Giedroc, D.P. 2008. Structural lability in stem-loop 1 drives a 5' UTR-3' UTR interaction in coronavirus replication. J Mol Biol 377: 790-803.
Liu, P., Millership, J.J., Li, L., Giedroc, D.P., and Leibowitz, J.L. 2006. A previously unrecognized UNR stem-loop structure in the coronavirus 5' untranslated region plays a functional role in replication. Adv Exp Med Biol 581: 25-30.
Kang, H., Feng, M., Schroeder, M.E., Giedroc, D.P., and Leibowitz, J.L. 2006. Putative cis-acting stem-loops in the 5' untranslated region of the severe acute respiratory syndrome coronavirus can substitute for their mouse hepatitis virus counterparts. J Virol 80: 10600-10614.
Kang, H., Feng, M., Schroeder, M.E., Giedroc, D.P., and Leibowitz, J.L. 2006. Stem-loop 1 in the 5' UTR of the SARS coronavirus can substitute for its counterpart in mouse hepatitis virus. Adv Exp Med Biol 581: 105-108.
De Albuquerque, N., Baig, E., Ma, X., Zhang, J., He, W., Rowe, A., Habal, M., Liu, M., Shalev, I., Downey, G.P. et al. 2006. Murine hepatitis virus strain 1 produces a clinically relevant model of severe acute respiratory syndrome in A/J mice. J Virol 80: 10382-10394.